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Cd22 ELISA Kit (Mouse)

CAT: 0247-OKEH04583-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04583-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
CD22 antigen
Gene Aliases
A530093D23; B-cell receptor CD22; BL-CAM; B-lymphocyte cell adhesion molecule; Lyb; Lyb-; Lyb8; Lyb-8; sialic acid-binding Ig-like lectin 2; siglec-2; T-cell surface antigen Leu-14.
Gene ID
12483
Accession Number
NP_001036782.1
Reactivity
Mouse, Mus musculus
Target
B-cell receptor CD22
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Cd22 ELISA Kit (Mouse) (OKEH04583) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cd22 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Cd22 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cd22 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
97 KDa
Specificity
Natural and recombinant Mouse B-cell receptor CD22
Protein Length
862
NCBI Gene Symbol
Cd22
Protein Name
P35329
Gene Name URL
Cd22
Nucleotide Accession Number
NM_001043317.2

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