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SPINK1 ELISA Kit (Human)

CAT: 0247-OKEH04577-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04577-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Serine peptidase inhibitor Kazal type 1
Gene Aliases
Pancreatic secretory trypsin inhibitor; PCTT; PSTI; serine protease inhibitor Kazal-type 1; serine protease inhibitor, Kazal type 1; Spink3; TATI; TCP; tumor-associated trypsin inhibitor.
Gene ID
6690
Accession Number
NP_003113.2
Reactivity
Homo sapiens, Human
Target
Serine protease inhibitor Kazal-type 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SPINK1 ELISA Kit (Human) (OKEH04577) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SPINK1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for SPINK1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SPINK1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 102%
Sensitivity
0.13 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
9 KDa
Specificity
Natural and recombinant Human Serine protease inhibitor Kazal-type 1
Protein Length
79
NCBI Gene Symbol
SPINK1
Protein Name
P00995
Gene Name URL
SPINK1
Nucleotide Accession Number
NM_003122.4

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