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Il15ra ELISA Kit (Mouse)

CAT: 0247-OKEH04565-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04565-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Interleukin 15 receptor, alpha chain
Gene Aliases
AA690181; IL-15 receptor subunit alpha; IL-15RA; IL-15R-alpha; interleukin-15 receptor subunit alpha.
Gene ID
16169
Accession Number
NP_001258426.1
Reactivity
Mouse, Mus musculus
Target
Interleukin-15 receptor subunit alpha
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Il15ra ELISA Kit (Mouse) (OKEH04565) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Il15ra has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Il15ra is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Il15ra captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Sensitivity
39.6 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
28 KDa
Specificity
Natural and recombinant Mouse Interleukin-15 receptor subunit alpha
Protein Length
263
NCBI Gene Symbol
Il15ra
Protein Name
Q60819
Gene Name URL
Il15ra
Nucleotide Accession Number
NM_001271497.1

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