Ctsb ELISA Kit (Mouse)

CAT: 0247-OKEH04554-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH04554-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Cathepsin B
Gene Aliases
Cathepsin B; cathepsin B1; CB.
Gene ID
13030
Accession Number
NP_031824.1
Reactivity
Mouse, Mus musculus
Target
Cathepsin B
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ctsb ELISA Kit (Mouse) (OKEH04554) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ctsb has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Ctsb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ctsb captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.78-50ng/mL
Sensitivity
0.422 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
37 KDa
Specificity
Natural and recombinant Mouse Cathepsin B
Protein Length
339
NCBI Gene Symbol
Ctsb
Protein Name
P10605
Gene Name URL
Ctsb
Nucleotide Accession Number
NM_007798.3

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