Ccl2 ELISA Kit (Mouse)

CAT: 0247-OKEH04536-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH04536-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Chemokine (C-C motif) ligand 2
Gene Aliases
AI323594; C-C motif chemokine 2; HC11; JE; MCA; MCAF; MCP; MCP-; MCP1; MCP-1; monocyte chemoattractant protein 1; monocyte chemotactic protein 1; platelet-derived growth factor-inducible protein JE; Scy; Scya2; Sig; Sigje; small-inducible cytokine A2; SMC-C; SMC-CF.
Gene ID
20296
Accession Number
NP_035463.1
Reactivity
Mouse, Mus musculus
Target
C-C motif chemokine 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ccl2 ELISA Kit (Mouse) (OKEH04536) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ccl2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Ccl2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ccl2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
31.2-2000pg/mL
Sensitivity
15.74 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
16 KDa
Specificity
Natural and recombinant Mouse C-C motif chemokine 2
Protein Length
148
NCBI Gene Symbol
Ccl2
Protein Name
P10148
Gene Name URL
Ccl2
Nucleotide Accession Number
NM_011333.3

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