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Tnfsf11 ELISA Kit (Mouse)

CAT: 0247-OKEH04531-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04531-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Tumor necrosis factor (ligand) superfamily, member 11
Gene Aliases
Ly10; Ly109l; O; OD; ODF; OPG ligand; OPGL; osteoclast differentiation factor; osteoprotegerin ligand; R; RANKL; receptor activator of NF-kappaB ligand; receptor activator of nuclear factor kappa-B ligand; TNF-related activation-induced cytokine; Tra; Trance; tumor necrosis factor ligand superfamily member 11; tumor necrosis factor-related activation-induced cytokine.
Gene ID
21943
Accession Number
NP_035743.2
Reactivity
Mouse, Mus musculus
Target
Tumor necrosis factor ligand superfamily member 11
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Tnfsf11 ELISA Kit (Mouse) (OKEH04531) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tnfsf11 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Tnfsf11 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tnfsf11 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
12.5-800pg/mL
Sensitivity
7.92 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
35 KDa
Specificity
Natural and recombinant Mouse Tumor necrosis factor ligand superfamily member 11
Protein Length
316
NCBI Gene Symbol
Tnfsf11
Protein Name
O35235
Gene Name URL
Tnfsf11
Nucleotide Accession Number
NM_011613.3

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