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Il10 ELISA Kit (Rat)

CAT: 0247-OKEH04509-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04509-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Interleukin 10
Gene Aliases
CSIF; cytokine synthesis inhibitory factor; If2a; IL-10; IL10X; interferon 2a; interleukin-10.
Gene ID
25325
Accession Number
NP_036986.2
Reactivity
Rat, Rattus norvegicus
Target
Interleukin-10
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Il10 ELISA Kit (Rat) (OKEH04509) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Il10 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Il10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Il10 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
31.2-2000pg/mL
Sensitivity
15.67 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
20 KDa
Specificity
Natural and recombinant Rat Interleukin-10
Protein Length
178
NCBI Gene Symbol
Il10
Protein Name
P29456
Gene Name URL
Il10
Nucleotide Accession Number
NM_012854.2

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