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Icam1 ELISA Kit (Mouse)

CAT: 0247-OKEH04504-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04504-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Intercellular adhesion molecule 1
Gene Aliases
CD54; Icam; Icam-1; intercellular adhesion molecule 1; Ly-4; Ly-47; MAL; MALA-2; myD10.
Gene ID
15894
Accession Number
NP_034623.1
Reactivity
Mouse, Mus musculus
Target
Intercellular adhesion molecule 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Icam1 ELISA Kit (Mouse) (OKEH04504) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Icam1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Icam1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Icam1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
62.5-4000pg/mL
Sensitivity
31.26 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
59 KDa
Specificity
Natural and recombinant Mouse Intercellular adhesion molecule 1
Protein Length
537
NCBI Gene Symbol
Icam1
Protein Name
P13597
Gene Name URL
Icam1
Nucleotide Accession Number
NM_010493.2

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