Tshb ELISA Kit (Rat)

7732-18-5

Thyroid stimulating hormone subunit beta

Thyroid stimulating hormone, beta subunit; Thyroid-stimulating hormone subunit beta; thyrotropin beta chain; thyrotropin subunit beta; TSH-B; TSH-beta.

25653

NP_037248.2

Rat, Rattus norvegicus

Thyrotropin subunit beta

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Aviva Systems Biology Tshb ELISA Kit (Rat) (OKEH04496) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Rat Tshb antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Tshb and incubated. The RBL1 found in the sample or standards competes with the biotinylated Tshb for limited binding sites on the immobilized anti-Rat Tshb antibody. Excess unbound biotinylated Tshb and sample or standard Tshb is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Tshb captured in the well and inversely proportional to the amount of Tshb which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

0.312-20mIU/mL

0.156mIU/mL

Store as indicated in product manual.

15 KDa

Natural and recombinant Rat Thyrotropin subunit beta

138

Tshb

P04652

Tshb