Tnfrsf11a ELISA Kit (Mouse)

• Reproducibility:

  Mean Inter-assay CV%:

• Gene Name:

  Tumor necrosis factor receptor superfamily, member 11a, NFKB activator

• Gene Aliases:

  Ly109; mRANK; ODFR; OFE; osteoclast differentiation factor receptor; Rank; receptor activator of NF-kappaB; receptor activator of NF-KB; TRANCE-R; tumor necrosis factor receptor superfamily member 11A.

• Gene ID:

  21934

• Reactivity:

  Mouse, Mus musculus

• Target:

  Tumor necrosis factor receptor superfamily member 11A

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology Tnfrsf11a ELISA Kit (Mouse) (OKEH04489) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Tnfrsf11a has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and washed. After washing, a biotinylated detector antibody specific for Tnfrsf11a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tnfrsf11a captured in well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

• Detection Range:

  31.2-2000pg/mL

• Recovery:

  Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

• Sensitivity:

  15.62 pg/mL

• Reconstitution:

  Store as indicated in product manual.

• Molecular Weight:

  66.6 kDa

• Specificity:

  Natural and recombinant Mouse Tumor necrosis factor receptor superfamily member 11A

• Protein Length:

  625

• NCBI Gene Symbol:

  Tnfrsf11a

• Protein Name:

  O35305

• Gene Name URL:

  Tnfrsf11a

• CAS Number:

  7732-18-5