LAMC2 ELISA Kit (Human)

CAT: 0247-OKEH04464-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH04464-96WSize:96 Well
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Laminin subunit gamma 2
Gene Aliases
B2T; BM600; BM600-100kDa; cell-scattering factor 140 kDa subunit; CSF; CSF 140 kDa subunit; EBR2; EBR2A; epiligrin subunit gamma; kalinin subunit gamma; Kalinin/nicein/epiligrin 100 kDa subunit; ladsin 140 kDa subunit; LAMB2T; laminin B2t chain; laminin subunit gamma-2; laminin, gamma 2; Laminin-5 subunit gamma; LAMNB2; large adhesive scatter factor 140 kDa subunit; nicein subunit gamma.
Gene ID
3918
Accession Number
NP_005553.2
Reactivity
Homo sapiens, Human
Target
Laminin subunit gamma-2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology LAMC2 ELISA Kit (Human) (OKEH04464) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LAMC2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for LAMC2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LAMC2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
62.5-4000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
Sensitivity
21 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
131 KDa
Specificity
Natural and recombinant Human Laminin subunit gamma-2
Protein Length
1193
NCBI Gene Symbol
LAMC2
Protein Name
Q13753
Gene Name URL
LAMC2
Nucleotide Accession Number
NM_005562.2

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