CD52 ELISA Kit (Human)

CAT: 0247-OKEH04460-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH04460-96WSize:96 Well
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
CD52 molecule
Gene Aliases
Cambridge pathology 1 antigen; CAMPATH-1 antigen; CD52 antigen (CAMPATH-1 antigen) ; CDW52; CDW52 antigen (CAMPATH-1 antigen) ; EDDM5; epididymal secretory protein E5; epididymis secretory sperm binding protein Li 171mP; HE5; HEL-S-171mP; human epididymis-specific protein 5.
Gene ID
1043
Accession Number
NP_001794.2
Reactivity
Homo sapiens, Human
Target
CAMPATH-1 antigen
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CD52 ELISA Kit (Human) (OKEH04460) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD52 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for CD52 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD52 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 111%
Sensitivity
0.12 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
7 KDa
Notes
Formerly GWB-U9F2Q6
Specificity
Natural and recombinant Human CAMPATH-1 antigen
Protein Length
61
NCBI Gene Symbol
CD52
Protein Name
P31358
Gene Name URL
CD52
Nucleotide Accession Number
NM_001803.2

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