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PRG2 ELISA Kit (Human)

CAT: 0247-OKEH04426-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04426-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Proteoglycan 2, pro eosinophil major basic protein
Gene Aliases
BMPG; bone marrow proteoglycan; eosinophil granule major basic protein; eosinophil major basic protein; MBP; MBP1; natural killer cell activator; proMBP; Proteoglycan 2; proteoglycan 2 preproprotein; proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein) .
Gene ID
5553
Accession Number
NP_001230174.1
Reactivity
Homo sapiens, Human
Target
Bone marrow proteoglycan
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PRG2 ELISA Kit (Human) (OKEH04426) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PRG2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for PRG2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PRG2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 110%
Sensitivity
0.1 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
25 KDa
Specificity
Natural and recombinant Human Bone marrow proteoglycan
Protein Length
222
NCBI Gene Symbol
PRG2
Protein Name
P13727
Gene Name URL
PRG2
Nucleotide Accession Number
NM_001243245.2

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