SFTPD ELISA Kit (Rat)

CAT: 0247-OKEH04421-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH04421-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Surfactant protein D
Gene Aliases
CP4; lung surfactant protein D; PSP-D; pulmonary surfactant protein D; pulmonary surfactant-associated protein D; SPD; SP-D; surfactant associated protein D; surfactant, pulmonary-associated protein D.
Gene ID
25350
Accession Number
NP_037010.1
Reactivity
Rat, Rattus norvegicus
Target
Pulmonary surfactant-associated protein D
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Sftpd ELISA Kit (Rat) (OKEH04421) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Sftpd has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Sftpd is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Sftpd captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.087 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
38 KDa
Specificity
Natural and recombinant Rat Pulmonary surfactant-associated protein D
Protein Length
374
NCBI Gene Symbol
Sftpd
Protein Name
P35248
Gene Name URL
Sftpd
Nucleotide Accession Number
NM_012878.2

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