VLDLR ELISA Kit (Human)
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VLDLR ELISA Kit (Human)
Reproducibility :
Mean Inter-assay CV%:Gene Name :
Very low density lipoprotein receptorGene Aliases :
CAMRQ1; CARMQ1; CHRMQ1; very low-density lipoprotein receptor; VLDL receptor; VLDL-R; VLDLRCH.Gene ID :
7436Accession Number :
NP_001018066.1Reactivity :
Homo sapiens, HumanTarget :
Very low-density lipoprotein receptorType :
ELISA KitApplications :
Enzyme-linked Immunosorbent assay-SandwichAssay Principle :
Aviva Systems Biology VLDLR ELISA Kit (Human) (OKEH04406) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for VLDLR has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for VLDLR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample VLDLR captured in the well.Assay Protocol :
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) ProtocolSample Type :
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological FluidsDetection Range :
0.78-50ng/mLRecovery :
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%Sensitivity :
0.23 ng/mLReconstitution :
Store as indicated in product manual.Molecular Weight :
96 KDaSpecificity :
Natural and recombinant Human Very low-density lipoprotein receptorProtein Length :
873NCBI Gene Symbol :
VLDLRProtein Name :
P98155Gene Name URL :
VLDLRCAS Number :
7732-18-5Nucleotide Accession Number :
NM_001018056.2

