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TNMD ELISA Kit (Rat)

CAT: 0247-OKEH04398-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04398-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Tenomodulin
Gene Aliases
ChM1L; chondromodulin-1-like protein; chondromodulin-I-like protein; myodulin; rChM1L; rTeM; teM; tenomodulin.
Gene ID
64104
Accession Number
NP_071626.1
Reactivity
Rat, Rattus norvegicus
Target
Tenomodulin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology Tnmd ELISA Kit (Rat) (OKEH04398) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Rat Tnmd antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Tnmd and incubated. The found in the sample or standards competes with the biotinylated Tnmd for limited binding sites on the immobilized anti-Rat Tnmd antibody. Excess unbound biotinylated Tnmd and sample or standard Tnmd is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Tnmd captured in the well and inversely proportional to the amount of Tnmd which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.089 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
37 KDa
Specificity
Natural and recombinant Rat Tenomodulin
Protein Length
317
NCBI Gene Symbol
Tnmd
Protein Name
Q9ESC2
Gene Name URL
Tnmd
Nucleotide Accession Number
NM_022290.1

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