ABCG2 ELISA Kit (Mouse)

CAT: 0247-OKEH04395-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH04395-96WSize:96 Well
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
ATP binding cassette subfamily G member 2 (Junior blood group)
Gene Aliases
ABC15; ABCP; AI428558; ATP-binding cassette sub-family G member 2; ATP-binding cassette, sub-family G (WHITE), member 2; Bc; BCRP; Bcrp1; breast cancer resistance protein 1 homolog; broad substrate specificity ATP-binding cassette transporter ABCG2; mitoxantrone resistance protein 1; MXR; MXR1; urate exporter.
Gene ID
26357
Accession Number
NP_036050.1
Reactivity
Mouse, Mus musculus
Target
ATP-binding cassette sub-family G member 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology Abcg2 ELISA Kit (Mouse) (OKEH04395) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mouse Abcg2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Abcg2 and incubated. The found in the sample or standards competes with the biotinylated Abcg2 for limited binding sites on the immobilized anti-Mouse Abcg2 antibody. Excess unbound biotinylated Abcg2 and sample or standard Abcg2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Abcg2 captured in the well and inversely proportional to the amount of Abcg2 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
Sensitivity
0.165 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
73 KDa
Specificity
Natural and recombinant Mouse ATP-binding cassette sub-family G member 2
Protein Length
657
NCBI Gene Symbol
Abcg2
Protein Name
Q7TMS5
Gene Name URL
Abcg2
Nucleotide Accession Number
NM_011920.3

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