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SPARC ELISA Kit (Mouse)

CAT: 0247-OKEH04375-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04375-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Secreted acidic cysteine rich glycoprotein
Gene Aliases
Basement-membrane protein 40; BM-40; ON; osteo; osteonectin; secreted protein acidic and rich in cysteine; SPARC.
Gene ID
20692
Accession Number
NP_033268.1
Reactivity
Mouse, Mus musculus
Target
SPARC
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Sparc ELISA Kit (Mouse) (OKEH04375) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Sparc has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Sparc is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Sparc captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
3.12-200ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 116%
Sensitivity
1.68 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
34 KDa
Specificity
Natural and recombinant Mouse SPARC
Protein Length
302
NCBI Gene Symbol
Sparc
Protein Name
P07214
Gene Name URL
Sparc
Nucleotide Accession Number
NM_009242.5

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