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OAS2 ELISA Kit (Human)

CAT: 0247-OKEH04370-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04370-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
2'-5'-oligoadenylate synthetase 2
Gene Aliases
(2'-5') oligo (A) synthetase 2;2-5A synthase 2;2'-5'-oligoadenylate synthase 2;2'-5'-oligoadenylate synthetase 2, 69/71kDa; p69 OAS / p71 OAS.
Gene ID
4939
Accession Number
NP_001027903.1
Reactivity
Homo sapiens, Human
Target
2'-5'-oligoadenylate synthase 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology OAS2 ELISA Kit (Human) (OKEH04370) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human OAS2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated OAS2 and incubated. The found in the sample or standards competes with the biotinylated OAS2 for limited binding sites on the immobilized anti-Human OAS2 antibody. Excess unbound biotinylated OAS2 and sample or standard OAS2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated OAS2 captured in the well and inversely proportional to the amount of OAS2 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Sensitivity
0.12 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
82 KDa
Specificity
Natural and recombinant Human 2'-5'-oligoadenylate synthase 2
Protein Length
719
NCBI Gene Symbol
OAS2
Protein Name
P29728
Gene Name URL
OAS2
Nucleotide Accession Number
NM_001032731.1

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