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GCK ELISA Kit (Mouse)

CAT: 0247-OKEH04363-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04363-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Glucokinase
Gene Aliases
Gk; GLK; Gls0; Gls006; hexokinase type IV; hexokinase-4; hexokinase-D; HK IV; HK4; HKIV; Hlb6; Hlb62; HXKP; MODY; MODY2.
Gene ID
103988
Accession Number
NP_001274315.1
Reactivity
Mouse, Mus musculus
Target
Glucokinase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Gck ELISA Kit (Mouse) (OKEH04363) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gck has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Gck is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gck captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 111%
Sensitivity
39.8 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
52 KDa
Specificity
Natural and recombinant Mouse Glucokinase
Protein Length
465
NCBI Gene Symbol
Gck
Protein Name
P52792
Gene Name URL
Gck
Nucleotide Accession Number
NM_001287386.1

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