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CFH ELISA Kit (Mouse)

CAT: 0247-OKEH04360-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04360-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Complement component factor h
Gene Aliases
Complement factor H; complement factor H related protein 3A4/5G4; Mud-1; NOM; protein beta-1-H; S; Sa; Sas1; Sas-1.
Gene ID
12628
Accession Number
NP_034018.2
Reactivity
Mouse, Mus musculus
Target
Complement factor H
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Cfh ELISA Kit (Mouse) (OKEH04360) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cfh has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Cfh is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cfh captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
3.12-200ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%
Sensitivity
1.64 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
139 KDa
Specificity
Natural and recombinant Mouse Complement factor H
Protein Length
1234
NCBI Gene Symbol
Cfh
Protein Name
P06909
Gene Name URL
Cfh
Nucleotide Accession Number
NM_009888.3

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