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AQP3 ELISA Kit (Mouse)

CAT: 0247-OKEH04351-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04351-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Aquaporin 3
Gene Aliases
AQP-2; AQP-3; aquaglyceroporin-3; aquaporin-3.
Gene ID
11828
Accession Number
NP_057898.2
Reactivity
Mouse, Mus musculus
Target
Aquaporin-3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Aqp3 ELISA Kit (Mouse) (OKEH04351) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mouse Aqp3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Aqp3 and incubated. The found in the sample or standards competes with the biotinylated Aqp3 for limited binding sites on the immobilized anti-Mouse Aqp3 antibody. Excess unbound biotinylated Aqp3 and sample or standard Aqp3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Aqp3 captured in the well and inversely proportional to the amount of Aqp3 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
32 KDa
Specificity
Natural and recombinant Mouse Aquaporin-3
Protein Length
292
NCBI Gene Symbol
Aqp3
Protein Name
Q8R2N1
Gene Name URL
Aqp3
Nucleotide Accession Number
NM_016689.2

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