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VDR ELISA Kit (Mouse)

CAT: 0247-OKEH04325-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04325-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Vitamin D (1,25-dihydroxyvitamin D3) receptor
Gene Aliases
1,25-dihydroxyvitamin D3 receptor; Nr; Nr1i1; nuclear receptor subfamily 1 group I member 1; vitamin D receptor; vitamin D3 receptor.
Gene ID
22337
Accession Number
NP_033530.2
Reactivity
Mouse, Mus musculus
Target
Vitamin D3 receptor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Vdr ELISA Kit (Mouse) (OKEH04325) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Vdr has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Vdr is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Vdr captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
Sensitivity
0.161 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
48 kDa
Specificity
Natural and recombinant Mouse Vitamin D3 receptor
Protein Length
422
NCBI Gene Symbol
Vdr
Protein Name
P48281
Gene Name URL
Vdr
Nucleotide Accession Number
NM_009504.4

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