DUSP5 ELISA Kit (Human)

CAT: 0247-OKEH04315-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH04315-96WSize:96 Well
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Dual specificity phosphatase 5
Gene Aliases
Dual specificity protein phosphatase 5; dual specificity protein phosphatase hVH3; DUSP; HVH3; serine/threonine specific protein phosphatase; VH1-like phosphatase 3.
Gene ID
1847
Accession Number
NP_004410.3
Reactivity
Homo sapiens, Human
Target
Dual specificity protein phosphatase 5
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology DUSP5 ELISA Kit (Human) (OKEH04315) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DUSP5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DUSP5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DUSP5 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
Sensitivity
0.11 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
42 kDa
Specificity
Natural and recombinant Human Dual specificity protein phosphatase 5
Protein Length
384
NCBI Gene Symbol
DUSP5
Protein Name
Q16690
Gene Name URL
DUSP5
Nucleotide Accession Number
NM_004419.3

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