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CPT1A ELISA Kit (Rat)

CAT: 0247-OKEH04308-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04308-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Carnitine palmitoyltransferase 1A
Gene Aliases
Carnitine O-palmitoyltransferase 1, liver isoform; Carnitine O-palmitoyltransferase I, liver isoform; carnitine palmitoyltransferase 1, liver; Carnitine palmitoyltransferase 1A; carnitine palmitoyltransferase 1a, liver; CPT I; CPT1-L; CPT-Ia; CPTI-L.
Gene ID
25757
Accession Number
NP_113747.2
Reactivity
Rat, Rattus norvegicus
Target
Carnitine O-palmitoyltransferase 1, liver isoform
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Cpt1a ELISA Kit (Rat) (OKEH04308) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cpt1a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cpt1a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cpt1a captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
15.6-1000pg/mL
Sensitivity
7.8 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
88 kDa
Specificity
Natural and recombinant Rat Carnitine O-palmitoyltransferase 1, liver isoform
Protein Length
773
NCBI Gene Symbol
Cpt1a
Protein Name
P32198
Gene Name URL
Cpt1a
Nucleotide Accession Number
NM_031559.2

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