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GDF11 ELISA Kit (Mouse)

CAT: 0247-OKEH04302-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04302-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Growth differentiation factor 11
Gene Aliases
Bmp1; Bmp11; BMP-11; bone morphogenetic protein 11; GDF-11; growth/differentiation factor 11.
Gene ID
14561
Accession Number
NP_034402.1
Reactivity
Mouse, Mus musculus
Target
Growth/differentiation factor 11
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Gdf11 ELISA Kit (Mouse) (OKEH04302) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gdf11 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Gdf11 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gdf11 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 110%
Sensitivity
7.94 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
45 kDa
Specificity
Natural and recombinant Mouse Growth/differentiation factor 11
Protein Length
405
NCBI Gene Symbol
Gdf11
Protein Name
Q9Z1W4
Gene Name URL
Gdf11
Nucleotide Accession Number
NM_010272.1

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