CDC42 ELISA Kit (Human)

CAT: 0247-OKEH04288-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH04288-96WSize:96 Well
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Cell division cycle 42
Gene Aliases
CDC42Hs; cell division control protein 42 homolog; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD) ) ; dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD) ) ; G25K; G25K GTP-binding protein; growth-regulating protein; GTP binding protein, 25kDa; small GTP binding protein CDC42; TKS.
Gene ID
998
Accession Number
NP_001034891.1
Reactivity
Homo sapiens, Human
Target
Cell division control protein 42 homolog
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CDC42 ELISA Kit (Human) (OKEH04288) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CDC42 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CDC42 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CDC42 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
Sensitivity
10 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
21 kDa
Specificity
Natural and recombinant Human Cell division control protein 42 homolog
Protein Length
191
NCBI Gene Symbol
CDC42
Protein Name
P60953
Gene Name URL
CDC42
Nucleotide Accession Number
NM_001039802.1

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