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PPP1R1A ELISA Kit (Mouse)

CAT: 0247-OKEH04282-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04282-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Protein phosphatase 1, regulatory inhibitor subunit 1A
Gene Aliases
0610038N18Rik; I-; I-1; inhib; inhibitor-1; IPP-1; protein phosphatase 1 regulatory subunit 1A; protein phosphatase inhibitor 1.
Gene ID
58200
Accession Number
NP_067366.1
Reactivity
Mouse, Mus musculus
Target
Protein phosphatase 1 regulatory subunit 1A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ppp1r1a ELISA Kit (Mouse) (OKEH04282) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ppp1r1a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ppp1r1a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ppp1r1a captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.156-10ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
Sensitivity
0.09 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
19 kDa
Specificity
Natural and recombinant Mouse Protein phosphatase 1 regulatory subunit 1A
Protein Length
171
NCBI Gene Symbol
Ppp1r1a
Protein Name
Q9ERT9
Gene Name URL
Ppp1r1a
Nucleotide Accession Number
NM_021391.3

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