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MEP1B ELISA Kit (Rat)

CAT: 0247-OKEH04269-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04269-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Meprin A subunit beta
Gene Aliases
Endopeptidase-2; meprin 1 beta; meprin A beta; meprin A subunit beta; meprin B.
Gene ID
25727
Accession Number
NP_037315.1
Reactivity
Rat, Rattus norvegicus
Target
Meprin A subunit beta
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Mep1b ELISA Kit (Rat) (OKEH04269) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mep1b has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Mep1b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mep1b captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.78-50ng/ml
Sensitivity
0.405 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
79 kDa
Specificity
Natural and recombinant Rat Meprin A subunit beta
Protein Length
704
NCBI Gene Symbol
Mep1b
Protein Name
P28826
Gene Name URL
Mep1b
Nucleotide Accession Number
NM_013183.2

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