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CYP21 ELISA Kit (Mouse)

CAT: 0247-OKEH04241-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04241-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Cytochrome P450, family 21, subfamily a, polypeptide 1
Gene Aliases
21-hydroxylase;21O;21-O;21OH;21-OH;21OHA;21-OHase;21OHB; Cyp2; Cyp21; Cyp21a2ps; Cyp21a2-ps; Cyp21B; CYP21OH-A; Cyp21-ps1; cytochrome P-450; cytochrome P450 21; cytochrome P450 hydroxylase A; cytochrome P450 XXI; cytochrome P450, 21, pseudogene; cytochrome P450, 21, steroid 21 hydroxylase; cytochrome P450, family 21, subfamily a, polypeptide 2, pseudogene; cytochrome P-450c21; cytochrome P450-C21; Oh21-; Oh21-1; Oh21-2; steroid 21-hydroxylase.
Gene ID
13079
Accession Number
NP_034125.2
Reactivity
Mouse, Mus musculus
Target
Steroid 21-hydroxylase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Cyp21 ELISA Kit (Mouse) (OKEH04241) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cyp21 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cyp21 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cyp21 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.156-10ng/ml
Recovery
<= 6.3%
Sensitivity
0.09 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
55 kDa
Specificity
Natural and recombinant Mouse Steroid 21-hydroxylase
Protein Length
487
NCBI Gene Symbol
Cyp21a1
Protein Name
P03940
Gene Name URL
Cyp21a1
Nucleotide Accession Number
NM_009995.2

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