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NMES1 ELISA Kit (Rat)

CAT: 0247-OKEH04238-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04238-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Hypothetical LOC302884
Gene Aliases
Nmes1; normal mucosa of esophagus-specific gene 1 protein.
Gene ID
302884
Accession Number
NP_001008518.1
Reactivity
Rat, Rattus norvegicus
Target
Normal mucosa of esophagus-specific gene 1 protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Nmes1 ELISA Kit (Rat) (OKEH04238) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nmes1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nmes1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nmes1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.312-20ng/ml
Sensitivity
0.174 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
10 kDa
Specificity
Natural and recombinant Rat Normal mucosa of esophagus-specific gene 1 protein
Protein Length
83
NCBI Gene Symbol
MGC105649
Protein Name
Q5RK28
Gene Name URL
MGC105649
Nucleotide Accession Number
NM_001008518.2

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