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DUT ELISA Kit (Rat)

CAT: 0247-OKEH04234-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04234-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Deoxyuridine triphosphatase
Gene Aliases
Deoxyuridine 5'-triphosphate nucleotidohydrolase; Dutp; dUTP pyrophosphatase; dUTPase; PIP4; PPAR-interacting protein 4.
Gene ID
497778
Accession Number
NP_001035361.1
Reactivity
Rat, Rattus norvegicus
Target
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Dut ELISA Kit (Rat) (OKEH04234) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dut has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Dut is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dut captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
78-5000pg/ml
Sensitivity
40 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
22 kDa
Specificity
Natural and recombinant Rat Deoxyuridine 5'-triphosphate nucleotidohydrolase
Protein Length
205
NCBI Gene Symbol
Dut
Protein Name
P70583
Gene Name URL
Dut
Nucleotide Accession Number
NM_001040271.1

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