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CTH ELISA Kit (Rat)

CAT: 0247-OKEH04230-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04230-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Cystathionine gamma-lyase
Gene Aliases
CTL target antigen; cystathionase (cystathionine gamma-lyase) ; cystathionine gamma-lyase; cysteine-protein sulfhydrase; gamma-cystathionase; H; PRB-RA; probasin-related antigen.
Gene ID
24962
Accession Number
NP_058770.1
Reactivity
Rat, Rattus norvegicus
Target
Cystathionine gamma-lyase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Cth ELISA Kit (Rat) (OKEH04230) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cth has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cth is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cth captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.78-50ng/ml
Sensitivity
0.399 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
44 kDa
Specificity
Natural and recombinant Rat Cystathionine gamma-lyase
Protein Length
398
NCBI Gene Symbol
Cth
Protein Name
P18757
Gene Name URL
Cth
Nucleotide Accession Number
NM_017074.1

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