NLRC4 ELISA Kit (Mouse)

CAT: 0247-OKEH04227-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH04227-96WSize:96 Well
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
NLR family, CARD domain containing 4
Gene Aliases
9530011P19Rik; Card; Card12; caspase recruitment domain family, member 12; caspase recruitment domain-containing protein 12; CLAN; CLAN1; CLANA; CLANB; CLANC; CLAND; I; ice protease-activating factor; IPAF; NLR family CARD domain-containing protein 4.
Gene ID
268973
Accession Number
NP_001028539.1
Reactivity
Mouse, Mus musculus
Target
NLR family CARD domain-containing protein 4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Nlrc4 ELISA Kit (Mouse) (OKEH04227) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nlrc4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nlrc4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nlrc4 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.156-10ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Sensitivity
0.083 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
117 kDa
Specificity
Natural and recombinant Mouse NLR family CARD domain-containing protein 4
Protein Length
1024
NCBI Gene Symbol
Nlrc4
Protein Name
Q3UP24
Gene Name URL
Nlrc4
Nucleotide Accession Number
NM_001033367.3