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NAIP ELISA Kit (Human)

CAT: 0247-OKEH04217-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04217-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
NLR family apoptosis inhibitory protein
Gene Aliases
Baculoviral IAP repeat-containing protein 1; BIRC1; neuronal apoptosis inhibitory protein; NLRB1; nucleotide-binding oligomerization domain, leucine rich repeat and BIR domain containing 1; psi neuronal apoptosis inhibitory protein; psiNAIP.
Gene ID
4671
Accession Number
NP_004527.2
Reactivity
Homo sapiens, Human
Target
Baculoviral IAP repeat-containing protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology NAIP ELISA Kit (Human) (OKEH04217) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NAIP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NAIP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NAIP captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
160 kDa
Specificity
Natural and recombinant Human Baculoviral IAP repeat-containing protein 1
Protein Length
1403
NCBI Gene Symbol
NAIP
Protein Name
Q13075
Gene Name URL
NAIP
Nucleotide Accession Number
NM_004536.2

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