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LRRK2 ELISA Kit (Human)

CAT: 0247-OKEH04209-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04209-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Leucine rich repeat kinase 2
Gene Aliases
Augmented in rheumatoid arthritis 17; AURA17; DARDARIN; leucine-rich repeat serine/threonine-protein kinase 2; PARK8; RIPK7; ROCO2.
Gene ID
120892
Accession Number
NP_940980.3
Reactivity
Homo sapiens, Human
Target
Leucine-rich repeat serine/threonine-protein kinase 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology LRRK2 ELISA Kit (Human) (OKEH04209) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LRRK2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LRRK2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LRRK2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.156-10ng/mL
Recovery
<= 4.7%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
286 kDa
Specificity
Natural and recombinant Human Leucine-rich repeat serine/threonine-protein kinase 2
Protein Length
2527
NCBI Gene Symbol
LRRK2
Protein Name
Q5S007
Gene Name URL
LRRK2
Nucleotide Accession Number
NM_198578.3

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