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HPGDS ELISA Kit (Mouse)

CAT: 0247-OKEH04201-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04201-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Hematopoietic prostaglandin D synthase
Gene Aliases
Glutathione S-transferase; glutathione-dependent PGD synthase; glutathione-dependent PGD synthetase; glutathione-requiring prostaglandin D synthase; GST class-sigma; hematopoietic prostaglandin D synthase; H-PG; H-PGDS; prostaglandin D2 synthase 2, hematopoietic; prostaglandin-H2 D-isomerase; Ptgd; Ptgds2.
Gene ID
54486
Accession Number
NP_062328.3
Reactivity
Mouse, Mus musculus
Target
Hematopoietic prostaglandin D synthase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology Hpgds ELISA Kit (Mouse) (OKEH04201) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-mouse Hpgds antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Hpgds and incubated. The Hpgds found in the sample or standards competes with the biotinylated Hpgds for limited binding sites on the immobilized anti-mouse Hpgds antibody. Excess unbound biotinylated Hpgds and sample or standard Hpgds is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Hpgds captured in the well and inversely proportional to the amount of Hpgds which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.625-40ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Sensitivity
0.324 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
23 kDa
Specificity
Natural and recombinant Mouse Hematopoietic prostaglandin D synthase
Protein Length
199
NCBI Gene Symbol
Hpgds
Protein Name
Q9JHF7
Gene Name URL
Hpgds
Nucleotide Accession Number
NM_019455.4

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