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FLT3 ELISA Kit (Mouse)

CAT: 0247-OKEH04097-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04097-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
FMS-like tyrosine kinase 3
Gene Aliases
B230315G04; CD135; fetal liver kinase 2; FL cytokine receptor; Flk; Flk-; Flk2; Flk-2; Flt-; Flt-3; Fms-like tyrosine kinase 3; Ly72; receptor-type tyrosine-protein kinase FLT3; tyrosine-protein kinase FLT3; tyrosine-protein kinase receptor flk-2; wmf; wmfl.
Gene ID
14255
Accession Number
NP_034359.2
Reactivity
Mouse, Mus musculus
Target
Receptor-type tyrosine-protein kinase FLT3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Flt3 ELISA Kit (Mouse) (OKEH04097) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Flt3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Flt3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Flt3 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108%
Sensitivity
15.63 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
113 kDa
Specificity
Natural and recombinant Mouse Receptor-type tyrosine-protein kinase FLT3
Protein Length
1000
NCBI Gene Symbol
Flt3
Protein Name
Q00342
Gene Name URL
Flt3
Nucleotide Accession Number
NM_010229.2

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