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KITLG ELISA Kit (Mouse)

CAT: 0247-OKEH04054-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04054-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Kit ligand
Gene Aliases
Bl; blz; C-kit ligand; Clo; cloud gray; Con; contrasted; Gb; grizzle-belly; hematopoietic growth factor KL; Kit; kit ligand; Kitlg; mast cell growth factor; Mgf; S; SC; SCF; SF; Sl; SLF; Ste; Steel factor; stem cell factor.
Gene ID
17311
Accession Number
NP_038626.1
Reactivity
Mouse, Mus musculus
Target
Kit ligand
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Kitlg ELISA Kit (Mouse) (OKEH04054) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Kitlg has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Kitlg is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Kitlg captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.078-5ng/ml
Sensitivity
0.039 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
31 kDa
Specificity
Natural and recombinant Mouse Kit ligand
Protein Length
273
NCBI Gene Symbol
Kitl
Protein Name
P20826
Gene Name URL
Kitl
Nucleotide Accession Number
NM_013598.2

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