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CCL19 ELISA Kit (Mouse)

CAT: 0247-OKEH04049-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04049-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Chemokine (C-C motif) ligand 19, predicted gene 2023
Gene Aliases
C-C motif chemokine 19; Ccl19; chemokine CCL19; CKb11; EBI1 ligand chemokine; EBI-1 ligand chemokine; EBV-induced molecule 1 ligand chemokine; ELC; epstein-Barr virus-induced molecule 1 ligand chemokine; exodus; exodus-3; Gm2023; MIP3B; Scya; Scya19; small-inducible cytokine A19.
Gene ID
100039053, 24047
Accession Number
NP_036018.1
Reactivity
Mouse, Mus musculus
Target
C-C motif chemokine 19
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ccl19 ELISA Kit (Mouse) (OKEH04049) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ccl19 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ccl19 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ccl19 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%
Sensitivity
7.93 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
12 kDa
Specificity
Natural and recombinant Mouse C-C motif chemokine 19
Protein Length
108
NCBI Gene Symbol
Ccl19, Gm2023
Protein Name
O70460
Gene Name URL
Ccl19, Gm2023
Nucleotide Accession Number
NM_011888.2

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