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CCL20 ELISA Kit (Mouse)

CAT: 0247-OKEH04047-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04047-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Chemokine (C-C motif) ligand 20
Gene Aliases
Beta-chemokine exodus-1; CC chemokine LARC; CC chemokine ST38; C-C motif chemokine 20; CKb4; exodus; exodus-1; LARC; liver and activation-regulated chemokine; macrophage inflammatory protein 3 alpha; MI; MIP; MIP-3; MIP-3[a]; MIP3A; MIP-3A; MIP-3-alpha; Scya; Scya20; small inducible cytokine subfamily A20; small-inducible cytokine A20; ST3; ST38.
Gene ID
20297
Accession Number
NP_001153210.1
Reactivity
Mouse, Mus musculus
Target
C-C motif chemokine 20
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ccl20 ELISA Kit (Mouse) (OKEH04047) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ccl20 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ccl20 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ccl20 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
3.9-250pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
Sensitivity
1.97 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
11 kDa
Specificity
Natural and recombinant Mouse C-C motif chemokine 20
Protein Length
97
NCBI Gene Symbol
Ccl20
Protein Name
O89093
Gene Name URL
Ccl20
Nucleotide Accession Number
NM_001159738.1