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GRN ELISA Kit (Human)

CAT: 0247-OKEH04008-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04008-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Granulin precursor
Gene Aliases
Acrogranin; CLN11; epithelin; GEP; glycoprotein 88; glycoprotein of 88 Kda; GP88; granulin-epithelin; granulins; PC cell-derived growth factor; PCDGF; PEPI; PGRN; proepithelin; progranulin.
Gene ID
2896
Accession Number
NP_002078.1
Reactivity
Homo sapiens, Human
Target
Granulins
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology GRN ELISA Kit (Human) (OKEH04008) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GRN has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GRN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GRN captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
Sensitivity
32 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
64 kDa
Specificity
Natural and recombinant Human Granulins
Protein Length
593
NCBI Gene Symbol
GRN
Protein Name
P28799
Gene Name URL
GRN
Nucleotide Accession Number
NM_002087.3