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FTO ELISA Kit (Rat)

CAT: 0247-OKEH03710-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH03710-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
FTO, alpha-ketoglutarate dependent dioxygenase
Gene Aliases
Alpha-ketoglutarate-dependent dioxygenase FTO; fat mass and obesity associated; fat mass and obesity-associated protein; m6A (m) -demethylase FTO; mRNA (2'-O-methyladenosine-N (6) -) -demethylase FTO; mRNA N (6) -methyladenosine demethylase FTO; protein fto; RGD1305121; tRNA N1-methyl adenine demethylase FTO; U6 small nuclear RNA (2'-O-methyladenosine-N (6) -) -demethylase FTO; U6 small nuclear RNA N (6) -methyladenosine-demethylase FTO.
Gene ID
291905
Accession Number
NP_001034802.1
Reactivity
Rat, Rattus norvegicus
Target
Alpha-ketoglutarate-dependent dioxygenase FTO
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology FTO ELISA Kit (Rat) (OKEH03710) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fto has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Fto is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fto captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/ml
Sensitivity
0.086 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
58 kDa
Specificity
Natural and recombinant Rat Alpha-ketoglutarate-dependent dioxygenase FTO
Protein Length
502
NCBI Gene Symbol
Fto
Protein Name
Q2A121
Gene Name URL
Fto
Nucleotide Accession Number
NM_001039713.1

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