CTXII ELISA Kit (Mouse)

• Reproducibility:

  Mean Inter-assay CV%:

• Gene Name:

  C-telopeptide of Collagen alpha-1 (II) chain

• Reactivity:

  Mouse

• Target:

  In bone physiology, the C-terminal telopeptide (or more formally, carboxy-terminal collagen crosslinks, and known by the acronym CTX) is a serum biomarker used to measure the rate of bone turnover. It can be useful in assisting clinicians to determine a patient's nonsurgical treatment response as well as evaluate a patient's risk of developing complications during healing following surgical intervention. The test used to detect the CTX marker is called the Serum CrossLaps, and it is more specific to bone resorption than any other test currently available.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology CTXII ELISA Kit (Mouse) (OKEH03670) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CTXII has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for CTXII is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CTXII captured in well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

• Detection Range:

  0.156-10ng/ml

• Recovery:

  Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%

• Sensitivity:

  0.085 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Specificity:

  Natural and recombinant Mouse C-telopeptide of Collagen alpha-1 (II) chain

• NCBI Gene Symbol:

  CTXII

• Protein Name:

  C-telopeptide of Collagen alpha-1 (II) chain

• Gene Name URL:

  CTXII

• CAS Number:

  7732-18-5