MAPT ELISA Kit (Mouse)

CAT: 0247-OKEH01411-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH01411-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Microtubule-associated protein tau
Gene Aliases
AI413597; AW045860; microtubule-associated protein tau; Mta; Mtapt; neurofibrillary tangle protein; paired helical filament-tau; PHF-tau; Ta; Tau.
Gene ID
17762
Accession Number
NP_001033698.1
Reactivity
Mouse, Mus musculus
Target
Microtubule-associated protein tau
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MAPT ELISA Kit (Mouse) (OKEH01411) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MAPT has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MAPT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MAPT captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
15.6-1000pg/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 91%
Sensitivity
8.14 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
76 kDa
Specificity
Natural and recombinant Mouse Microtubule-associated protein tau
Protein Length
733
NCBI Gene Symbol
Mapt
Protein Name
P10637
Gene Name URL
Mapt
Nucleotide Accession Number
NM_001038609.2

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