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CEACAM1 ELISA Kit (Mouse)

CAT: 0247-OKEH03642-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH03642-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Carcinoembryonic antigen-related cell adhesion molecule 1
Gene Aliases
Bb-1; Bg; Bgp; Bgp1; BGP-1; biliary glycoprotein 1; biliary glycoprotein D; carcinoembryonic antigen 1; carcinoembryonic antigen 7; carcinoembryonic antigen-related cell adhesion molecule 1; Cc1; C-CA; C-CAM; CD66; CD66a; Cea; Cea-; Cea1; Cea-1; Cea7; Cea-7; CEA-related cell adhesion molecule 1; hepatitis virus (MHV-4) susceptibility; hepatitis virus receptor; Hv; Hv-; Hv2; Hv-2; mCEA; mCEA1; MHV; Mhv-; Mhv-1; MHVR; MHV-R; MHVR1; mmCGM1; mmCGM1a; mmCGM2; murine hepatitis virus receptor.
Gene ID
26365
Accession Number
NP_001034274.1
Reactivity
Mouse, Mus musculus
Target
Carcinoembryonic antigen-related cell adhesion molecule 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CEACAM1 ELISA Kit (Mouse) (OKEH03642) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ceacam1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Ceacam1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ceacam1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84%
Sensitivity
40.2 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
57 kDa
Specificity
Natural and recombinant Mouse Carcinoembryonic antigen-related cell adhesion molecule 1
Protein Length
521
NCBI Gene Symbol
Ceacam1
Protein Name
P31809
Gene Name URL
Ceacam1
Nucleotide Accession Number
NM_001039185.1

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