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ITIH3 ELISA Kit (Human)

CAT: 0247-OKEH03639-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH03639-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Inter-alpha-trypsin inhibitor heavy chain 3
Gene Aliases
H3P; inter-alpha (globulin) inhibitor H3; inter-alpha (globulin) inhibitor, H3 polypeptide; inter-alpha-inhibitor heavy chain 3; inter-alpha-trypsin inhibitor heavy chain H3; ITI heavy chain H3; ITI-HC3; pre-alpha (globulin) inhibitor, H3 polypeptide; serum-derived hyaluronan-associated protein; SHAP.
Gene ID
3699
Accession Number
NP_002208.3
Reactivity
Homo sapiens, Human
Target
Inter-alpha-trypsin inhibitor heavy chain H3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ITIH3 ELISA Kit (Human) (OKEH03639) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human ITIH3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ITIH3 and incubated. The ITIH3 found in the sample or standards competes with the biotinylated ITIH3 for limited binding sites on the immobilized anti-human ITIH3 antibody. Excess unbound biotinylated ITIH3 and sample or standard ITIH3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ITIH3 captured in the well and inversely proportional to the amount of ITIH3 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88.9%
Sensitivity
0.19 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
100 kDa
Specificity
Natural and recombinant Human Inter-alpha-trypsin inhibitor heavy chain H3
Protein Length
890
NCBI Gene Symbol
ITIH3
Protein Name
Q06033
Gene Name URL
ITIH3
Nucleotide Accession Number
NM_002217.3

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