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PARM1 ELISA Kit (Mouse)

CAT: 0247-OKEH03552-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH03552-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Prostate androgen-regulated mucin-like protein 1
Gene Aliases
2210012L08Rik;9130213B05Rik; PARM-1; prostate androgen-regulated mucin-like protein 1 homolog; prostatic androgen-repressed message-1; W91666.
Gene ID
231440
Accession Number
NP_663537.1
Reactivity
Mouse, Mus musculus
Target
Prostate androgen-regulated mucin-like protein 1 homolog
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PARM1 ELISA Kit (Mouse) (OKEH03552) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Parm1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Parm1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Parm1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
Sensitivity
0.395 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
31 kDa
Specificity
Natural and recombinant Mouse Prostate androgen-regulated mucin-like protein 1 homolog
Protein Length
296
NCBI Gene Symbol
Parm1
Protein Name
Q923D3
Gene Name URL
Parm1
Nucleotide Accession Number
NM_145562.2

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