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AOX1 ELISA Kit (Rat)

CAT: 0247-OKEH03524-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03524-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Aldehyde oxidase 1
Gene Aliases
Aldehyde oxidase (female form) ; aldehyde oxidase 1; azaheterocycle hydroxylase 1.
Gene ID
54349
Accession Number
NP_062236.2
Reactivity
Rat, Rattus norvegicus
Target
Aldehyde oxidase 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology AOX1 ELISA Kit (Rat) (OKEH03524) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Aox1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Aox1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Aox1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.78-50ng/mL
Sensitivity
0.393 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
147 kDa
Specificity
Natural and recombinant Rat Aldehyde oxidase 1
Protein Length
1333
NCBI Gene Symbol
Aox1
Protein Name
Q9Z0U5
Gene Name URL
Aox1
Nucleotide Accession Number
NM_019363.3