CTCF ELISA Kit (Mouse)

• Reproducibility:

  Mean Inter-assay CV%:

• Gene Name:

  CCCTC-binding factor

• Gene Aliases:

  11-zinc finger protein; AW108038; CCCTC-binding factor; CTCFL paralog; transcriptional repressor CTCF.

• Gene ID:

  13018

• Accession Number:

  NP_851839.1

• Reactivity:

  Mouse, Mus musculus

• Target:

  Transcriptional repressor CTCF

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Competitive

• Assay Principle:

  Aviva Systems Biology Ctcf ELISA Kit (Mouse) (OKEH03508) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mouse Ctcf antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Ctcf and incubated. The Ctcf found in the sample or standards competes with the biotinylated Ctcf for limited binding sites on the immobilized anti-Mouse Ctcf antibody. Excess unbound biotinylated Ctcf and sample or standard Ctcf is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Ctcf captured in the well and inversely proportional to the amount of Ctcf which was contained in the sample or standard.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

• Detection Range:

  78-5000pg/mL

• Recovery:

  Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%

• Sensitivity:

  39.3 pg/mL

• Reconstitution:

  Store as indicated in product manual.

• Molecular Weight:

  84 kDa

• Specificity:

  Natural and recombinant Mouse Transcriptional repressor CTCF

• Protein Length:

  736

• NCBI Gene Symbol:

  Ctcf

• Protein Name:

  Q61164

• Gene Name URL:

  Ctcf

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_181322.3